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Cloning and Expression
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Primary Cells, Cell Lines and Microorganisms
Cells and Microorganisms
Peripheral Blood Mononuclear Cells
Peripheral Blood Mononuclear Cells from ATCC
Be the first to write a review!
Citations:
(37)
Supplier Page
Supplier Page from
ATCC for
Peripheral Blood Mononuclear Cells
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Citations
Reviews
Product Specs
Item
Peripheral Blood Mononuclear Cells
Company
ATCC
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
PCS-800-011
Quantity
1
Type
Primary Cell
Species
Human
Tissue
Peripheral blood
Cell Type (standardized)
Mononuclear Cells
Tissue (standardized)
Blood
Applications
3D cell culture, Immunology, Toxicology, Immuno-oncology, Cell and gene therapy (CGT) development
Research Area
3D cell culture, Immunology, Toxicology, Immuno-oncology, Cell and gene therapy (CGT) development
Cell Type
mononuclear
Gender
Lot specific
Age
Lot specific
Disease
normal
Add to Compare List
ATCC
10801 University Boulevard
Manassas
,
Virginia
20110-2209
United States
Phone
: (703) 365-2700
Fax: (703) 365-2701
Company Profile
Website:
http://www.atcc.org
Citations (37)
(1)
An Engineered Probiotic Produces a Type III Interferon IFNL1 and Reduces Inflammations in in vitro Inflammatory Bowel Disease Models
ACS Biomaterials Science & Engineering
September 11, 2023
Koon Jiew Chua, Hua Ling, In Young Hwang, Hui Ling Lee, John C. March, Yung Seng Lee, Matthew Wook Chang
CO2 atmosphere. CD4+ T cells were isolated from human peripheral blood mononuclear cells (PBMCs,
ATCC
PCS-800-011
) using an EasySep Human CD4+ T Cell Isolation Kit according to the manufacturer’s protocol. Isolated
(2)
TAGAP expression influences CD4+ T cell differentiation, immune infiltration, and cytotoxicity in LUAD through the STAT pathway: implications for immunotherapy
Frontiers in Immunology
September 6, 2023
Zhanyu Xu, Tiaozhan Zheng, Zhiwen Zheng, Wei Jiang, Liuliu Huang, Kun Deng, Liqiang Yuan, Fanglu Qin, Yu Sun, Junqi Qin,
et al.
Cell culture
Peripheral blood mononuclear cells
(hPBMC) from healthy individuals were purchased from
ATCC
(
PCS-800-011
). The
Dynabeads® CD4 Positive Isolation Kit
(cat.
11331D
,
Invitrogen
™) was used for
cell sorting
, and
(3)
Epithelial-Immune Cell Crosstalk Determines the Activation of Immune Cells In Vitro by the Human Cathelicidin LL-37 at Low Physiological Concentrations
Biomolecules
August 28, 2023
Ivan V. Bogdanov, Maria A. Streltsova, Elena I. Kovalenko, Alexander M. Sapozhnikov, Pavel V. Panteleev, Tatiana V. Ovchinnikova
mononuclear cells (PBMCs) from a healthy donor were purchased from the American Type Culture Collection (
ATCC
PCS-800-011
). Blood
monocytes
were isolated from PBMCs by plastic adhesion [17]. Mature
monocyte
-derived
dendritic cells
(4)
Immunomodulatory Effects of the Pea Defensin Psd1 in the Caco-2/Immune Cells Co-Culture upon Candida albicans Infection
International Journal of Molecular Sciences
April 23, 2023
Ivan V. Bogdanov, Serafima I. Fateeva, Alexander D. Voropaev, Tatiana V. Ovchinnikova, Ekaterina I. Finkina
cells (PBMCs) collected from healthy donors were purchased from American Type Culture Collection (
ATCC
PCS-800-011
). Primary CD14+ blood
monocytes
were isolated from PBMCs by adherence according to standard
technique
(5)
Comparative and Temporal Characterization of LPS and Blue-Light-Induced TLR4 Signal Transduction and Gene Expression in Optogenetically Manipulated Endothelial Cells
Cells
February 22, 2023
Stierschneider A, Neuditschko B, Colleselli K, Hundsberger H, Herzog F, Wiesner C.
University of Graz), and primary peripheral blood mononuclear cells (PBMCs) (
ATCC
®, Manassas, VD, USA;
PCS-800-011
™) (RRID:CVCL_WR41) were cultivated in a huMEC medium (InSCREENex, Braunschweig, Germany; INS-ME-1012)
(6)
MTSS1 curtails lung adenocarcinoma immune evasion by promoting AIP4-mediated PD-L1 monoubiquitination and lysosomal degradation
Cell Discovery
February 21, 2023
Yuan Wang, Zhenchang Jia, Chenxi Liang, Yunfei He, Min Cong, Qiuyao Wu, Pu Tian, Dasa He, Xiang Miao, Beibei Sun,
et al.
To analyze
T cell
-mediated
tumor
cell killing, human T cells were activated by culturing
human
PBMC (
PCS-800-011
,
ATCC
) in ImmunoCult-XF
T cell
expansion medium (
10981
,
Stemcell
) with
ImmunoCult human CD3/CD28 T cell activator
(7)
RNA cytometry of single-cells using semi-permeable microcapsules
Nucleic Acids Research
January 11, 2023
Greta Leonaviciene, Linas Mazutis
centrifugation
steps were performed at 300 × g for 5 min at 4°C. Preparation of PBMCs PBMCs (
ATCC
,
PCS-800–011
) were thawed from liquid nitrogen using
RPMI 1640 Medium
supplemented with 10% fetal bovine serum. Thawed
(8)
Antimicrobial Activity and Immunomodulatory Properties of Acidocin A, the Pediocin-like Bacteriocin with the Non-Canonical Structure
Membranes (Basel)
December 11, 2022
Daria V. Antoshina, Sergey V. Balandin, Ivan V. Bogdanov, Maria A. Vershinina, Elvira V. Sheremeteva, Ilia Yu. Toropygin, Ekaterina I. Finkina, Tatiana V. Ovchinnikova
acute monocytic leukemia
cell line (THP-1) were purchased from American Type Culture Collection (
ATCC
PCS-800-011
and
ATCC
TIB-202
, respectively). PBMCs and
THP-1 cells
were seeded into 96-well plates at 2 × 106 and
(9)
Antifungal Activity, Structural Stability, and Immunomodulatory Effects on Human Immune Cells of Defensin from the Lentil Lens culinaris
Membranes (Basel)
August 31, 2022
Ekaterina I. Finkina, Ivan V. Bogdanov, Anastasia A. Ignatova, Marina D. Kanushkina, Ekaterina A. Egorova, Alexander D. Voropaev, Elena A. Stukacheva, Tatiana V. Ovchinnikova
cells (PBMCs) collected from a healthy donor were purchased from American Type Culture Collection (
ATCC
PCS-800-011
). CD4+ T-helper and CD8+ T cytotoxic cells were isolated from PBMCs by magnetic separation with mouse
(10)
A Novel Proline-Rich Cathelicidin from the Alpaca Vicugna pacos with Potency to Combat Antibiotic-Resistant Bacteria: Mechanism of Action and the Functional Role of the C-Terminal Region
Membranes (Basel)
May 12, 2022
Pavel V. Panteleev, Victoria N. Safronova, Roman N. Kruglikov, Ilia A. Bolosov, Ivan V. Bogdanov, Tatiana V. Ovchinnikova
peripheral blood mononuclear cells
(PBMC) collected from a healthy donor were purchased from American Type Culture Collection (
ATCC
PCS-800-011
), thawed, and seeded into a 96-well plate one day prior to the experiment at a density of 2 × 105 cells/well.
(11)
Immunomodulatory functions of human mesenchymal stromal cells are enhanced when cultured on HEP/COL multilayers supplemented with interferon-gamma
Materials Today Bio
January 1, 2022
Mahsa Haseli, David A. Castilla-Casadiego, Luis Pinzon-Herrera, Alexander Hillsley, Katherine A. Miranda-Munoz, Srikanth Sivaraman, Adrianne M. Rosales, Raj R. Rao, Jorge Almodovar
capacity,
human
primary
peripheral blood mononuclear cells
(PBMC), were purchased from ATCC (
ATCC
®
PCS¬800¬011
™ Part number:
302213
). Briefly, 62500 hMSCs cells/cm2 with and without IFN-γ treatment were seeded on
(12)
Preclinical validation of a live attenuated dermotropic Leishmania vaccine against vector transmitted fatal visceral leishmaniasis
Communications Biology
December 1, 2021
Subir Karmakar et al.
infection
BMCs of seven different healthy donors from non-endemic regions were collected from
ATCC
, USA (
PCS-800-011
™). The viability of the PBMC was >98%, as checked by the trypan blue dye exclusion method. PBMCs were
(13)
Characterizing the NLRP3 Inflammasome in Mood Disorders: Overview, Technical Development, and Measures of Peripheral Activation in Adolescent Patients
International Journal of Molecular Sciences
November 19, 2021
Xinyang Zhou, Shehan M. Fernando, Alexander Y. Pan, Rebecca Laposa, Kathryn R. Cullen, Bonnie Klimes-Dougan, Ana C. Andreazza
utilized PBMC from unaffected individuals. Frozen PBMC from healthy donors (
ATCC
, Manassas, VA, USA, #
PCS-800-011
) were thawed and plated onto glass coverslips treated with poly-D-lysine (Fisher Scientific, Waltham,
(14)
Therapeutic Potential of Chemically Modified, Synthetic, Triplex Peptide Nucleic Acid–Based Oncomir Inhibitors for Cancer Therapy
Cancer Research
November 15, 2021
Karishma Dhuri, Ravinder Reddy Gaddam, Ajit Vikram, Frank J. Slack, Raman Bahal
treatment control. Safety assessment by trypan blue
assay
Primary blood mononuclear cells (PBMC) (
ATCC
®
PCS-800–011
™) were purchased from ATCC (Virginia, USA). The cells were regularly tested for
mycoplasma
contamination
(15)
Functional characterization of the immunomodulatory properties of human urine-derived stem cells
Translational Andrology and Urology
September 1, 2021
Rongpei Wu, Melisa Soland, Guihua Liu, Yingai Shi, Chi Zhang, Yiming Tang, Graça Almeida-Porada, Yuanyuan Zhang
following studies.
Cell culture
Human peripheral blood mononuclear cells (PBMNCs, normal
human
,
ATCC
PCS-800-011
) from two different donors were purchased from ATCC (American Type Culture Collection, MD, USA). PBMNCs
(16)
Antiviral, Cytotoxic, and Antioxidant Activities of Three Edible Agaricomycetes Mushrooms: Pleurotus columbinus, Pleurotus sajor-caju, and Agaricus bisporus
Journal of Fungi
August 8, 2021
Shaza M. Elhusseiny, Taghrid S. El-Mahdy, Mohamed F. Awad, Nooran S. Elleboudy, Mohamed M. S. Farag, Khaled M. Aboshanab, Mahmoud A. Yassien
UK). 2.6. Cell Lines and Cultures Primary peripheral blood mononuclear cells (PBMCs), normal,
human
(
ATCC
®
PCS-800-011
™) were purchased from Vascera, Cairo, Egypt. The
cancer
cell lines
human
leukemia
(
CCRF-CEM
);
acute promyelocytic leukemia
(17)
Proteome Analysis and In Vitro Antiviral, Anticancer and Antioxidant Capacities of the Aqueous Extracts of Lentinula edodes and Pleurotus ostreatus Edible Mushrooms
Molecules
July 30, 2021
Shaza M. Elhusseiny, Taghrid S. El-Mahdy, Mohamed F. Awad, Nooran S. Elleboudy, Mohamed M. S. Farag, Mahmoud A. Yassein, Khaled M. Aboshanab
(Mokatam, Cairo, Egypt), while primary peripheral blood mononuclear cells (PBMCs) and normal
human
(
ATCC
®
PCS-800-011
™) cells were purchased from Vascera, Cairo, Egypt. This work was approved by the Ethical Research Committee
(18)
A GATA3 Targeting Nucleic Acid Nanocapsule for
In Vivo
Gene Regulation in Asthma
ACS Nano
July 27, 2021
Tyler D. Gavitt, Alyssa K. Hartmann, Shraddha S. Sawant, Arlind B. Mara, Steven M. Szczepanek, Jessica L. Rouge
for p < 0.05. Cell Distribution
Flow Cytometry
.
Human
PBMCs were purchased from
ATCC
(catalog no.
PCS-800-011
). One million cells were aliquoted into each sample group and resuspended in FACS buffer. Groups receiving
(19)
Human macrophages utilize a wide range of pathogen recognition receptors to recognize Legionella pneumophila, including Toll-Like Receptor 4 engaging Legionella lipopolysaccharide and the Toll-like Receptor 3 nucleic-acid sensor
PLoS pathogens
July 1, 2021
Grigoryeva LS, Cianciotto NP.
CCL-240
),
human
primary
macrophages
derived from
peripheral blood mononuclear cells
(PBMC) (
ATCC
, #
PCS-800-011
), and murine primary
macrophages
derived from either the PBMCs or
bone
marrow (BMDMs) obtained from
(20)
Investigation of Sensitization Potential of the Soybean Allergen Gly m 4 by Using Caco-2/Immune Cells Co-Culture Model
Nutrients
June 16, 2021
Ivan V. Bogdanov, Ekaterina I. Finkina, Daria N. Melnikova, Rustam H. Ziganshin, Tatiana V. Ovchinnikova
peripheral blood mononuclear cells
(PBMC) collected from healthy donor were purchased from American Type Culture Collection (
ATCC
PCS-800-011
), thawed, and seeded into wells of 24- and 96-well plates 2 days prior to the experiment. Two different
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